http://news.bbc.co.uk/hi/english/health/newsid_2134000/2134624.stm

Life's First Moments Unravelled

Wednesday, 17 July, 2002, 16:50 GMT 17:50 UK '

BBC News
 

A developing embryo - but what sparks its growth

 

A gene that plays a key role in the first stage of embryonic life has been discovered by UK researchers.

 

It could hold the key to new male contraceptives - and help some couples who have been unable to have children.

The finding could also produce new, more efficient ways of cloning.


 

For the last 10 or 12 years we have been focusing specifically on identifying a molecule such as this


 

Professor Tony Lai, University of Wales College of Medicine

While scientists know that a sperm must burrow into the egg in order to fertilise, little is understood about the process that then ensues.

They know that cell division is triggered by a mysterious "wave" of calcium which surges through the egg, but until now, did not know how this happened.

 

Professor Tony Lai and a team of researchers at the University of Wales College of Medicine have indentified a "sperm factor" gene.

 

'Significant implications'

 

This produces a protein called PLC-zeta which appears to be key to producing the calcium reaction in the egg.

 

Professor Lai said: "For the last 10 or 12 years we have been focusing specifically on identifying a molecule such as this.

 

"Now we've found that this single molecule kicks the whole process off. It has very significant implications."

 

Those may include effective contraceptives for men which work by blocking the gene.

 

The team believes that if the PLC-zeta was absent, fertilisation could not take place.

 

To test the theory, it introduced the protein into single eggs - and watched as they started to divide and carry on developing right up to the "blastocyst" stage - small clusters of dozens of cells.

 

The technique has real potential to help scientists involved in cloning.

 

At the moment, to start cell division, the egg is given an electric shock.

 

This makes holes in the cell membrane through which calcium enters and simulates the surge, starting division.

 

But it is a crude technique that often fails.

 

Professor Lai said: "That's like banging it with a hammer and hoping it works. We hope that our molecule will make the process far more efficient and reliable."

 

Experts impressed

 

Professor Ian Wilmut, who led the team that created Dolly the sheep, agreed, saying he would certainly like to try the new protein.

 

"People have been looking for this for a long time, and they seem definitely to have found it."

 

The discovery could also help those who want to use embryonic stem cells as potential therapies for disease.